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Its phenotype is + bio + th – liu – met.
Lederberg and Totem scattered the two mutants and
They were trained for several hours in a medium containing the addition of methionine, biotin, threonine and leucine.

Both strains can develop. Then the bacteria were removed
Centrifugal, washed, replaced by medium
In very small medium, it is absent from all four complements. strain 1 or strain 2 cannot grow in this medium
the nutrition they need. However, some bacteria
Colonies appeared on culture plates.

They are small to medium, growing in these colonies.
+ Bio + Thr + Leu + must contain bacteria found; vehicle
Yes, they have to be prototropic. These colonies first appeared at a rate of approximately one for every 10 million cells placed in the tray (1/107).

Where did these prototropic colonies come from? Lederberg and Totem were able to eliminate the mutation, and so on. Investigators blocked this change (we discussed this process in Chapter 11, which we’ll look at in more detail

 

 

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